Use Guide to Electrophoresis

Use method of electrophoresis apparatus power supply:
Simply put, the power supply of electrophoresis apparatus can be divided into two types: compact type and multi-use type. The compact type is bistable, and the multi-use type is tri-stable (tri-constant) type. No matter there are several stability functions, the power supply of the electrophoresis apparatus can only stabilize some of the parameters in the actual work. As to which parameters to stabilize, it depends on the setting of the power supply of the electrophoresis apparatus and the equivalent load resistance of the electrophoresis apparatus. The power supply of electrophoresis apparatus sold on the market can be divided into three types according to voltage: voltage 1500 ~ 5000V, medium voltage 500 ~ 1500V, low voltage 500V below; According to the current, it is divided into three kinds: large current 500mA ~ 2000mA, medium current 100mA ~ 500mA, small current 100mA below; According to the power, it is divided into three kinds: high power 200 ~ 400W, ** rate 60 ~ 200W, and low power less than 60W. From the perspective of voltage, only 300V voltage is needed for nucleic acid (agarose) horizontal electrophoresis, PCR electrophoresis, DNA recovery, imprinting transfer and other experiments. Used for protein vertical electrophoresis, seed purity electrophoresis, acetate fiber membrane electrophoresis, etc., need to use voltage about 600V electrophoresis apparatus; For DNA sequencing (SSR molecular marker), isopoint focusing electrophoresis, two-dimensional electrophoresis and other requirements of voltage 3000V; Electrophoresis for DNA sequencing (AFLP molecular marker) requires a voltage of 3800V. All parameters of the power output of the electrophoresis apparatus have a specific working range according to its model. If the actual need exceeds this range, another type of power supply that can meet the use of the electrophoresis apparatus should be selected. Because of the different internal structure characteristics of the l