Application of agarose gel electrophoresis

Agarose gel electrophoresis is a routine method for isolating proteins, DNA or RNA.
Estimates of the size of DNA molecules
Analyze PCR products, for example in molecular genetic diagnosis or genetic fingerprinting
The restriction genomic DNA was isolated prior to Southern analysis, and the RNA was isolated prior to Northern analysis.
Agarose gel electrophoresis is widely used to assess the size of DNA fragments after restriction enzyme digestion, for example in the restriction profiles of cloned DNA.
Agarose gel electrophoresis is commonly used to parse circular DNA with different superhelical topologies and to parse segments that differ due to DNA synthesis.
In addition to providing an excellent medium for fragment size analysis, agarose gels can also purify DNA fragments. Since purification of DNA fragments isolated in agarose gels is required for many molecular techniques, such as cloning, being able to purify target fragments from the gels is critical.