How to use the Micro Spectrophotometer and what are the considerations?
A: Micro Spectrophotometer is a precision optical instrument, which is carefully assembled and debugged before leaving the factory. If the instrument can be properly maintained, it can not only guarantee the reliability and stability of the instrument, but also extend the service life of the instrument.
B: Micro Spectrophotometer is commonly used to quantify oligonucleotides, single-stranded, double-stranded DNA, and RNA soluble in buffers. The maximum absorption wavelength of nucleic acid is 260 nm. Each nucleic acid has a different molecular composition, so its conversion coefficient is different. To quantify different types of nucleic acids, the corresponding coefficients should be selected in advance. For example, the absorption value of 1OD is equivalent to 50μg/ mL of dsDNA, 37μg/ mL of ssDNA, 40μg/ mL of RNA, 30μg/ mL of OLIG. The absorption value after the test is converted by the above coefficients to obtain the corresponding sample concentration.
C: The working principle of Micro Spectrophotometer is an instrument for quantitative and qualitative analysis of substances by spectrophotometry. It is often used for the quantitative determination of nucleic acid, protein and bacterial growth concentration. Whether in physics, chemistry, biology, medicine, material science, environmental science and other scientific research fields, or in chemical industry, medicine, environmental detection, metallurgy and other modern production and management departments, UV visible spectrophotometer has a wide range of important applications.
D: Micro Spectrophotometer is a precision optical instrument, and proper installation, use and maintenance play an important role in maintaining the good performance of the instrument and ensuring the accuracy of the test. Now let's share some common sense of maintenance of the Micro Spectrophotometer.
(1) Requirements for the working environment of the instrument
The requirements for the working environment of the spectrophotometer are as follows.
① The instrument should be placed in a dry room, the operating temperature is 5 ~ 35℃, and the relative humidity should not exceed 85%.
② The instrument should be placed on a firm and stable workbench, and avoid strong vibration or continuous vibration.
③indoor lighting should not be too strong, and should avoid direct sunlight.
④ The fan should not blow directly to the instrument to prevent the light source lamp from affecting the normal use of the instrument because of the light instability.
⑤Try to stay away from high intensity magnetic field, electric field and high-frequency electrical equipment.
The power supply voltage of the instrument is AC 220V±22V, frequency is 50Hz±1Hz, and must be equipped with a good grounding wire. It is recommended to use electronic AC regulator or AC constant voltage regulator with power of more than 1000W to enhance the anti-interference performance of the instrument.
Avoid use in places with corrosive gases such as hydrogen sulfide.
(2) Routine maintenance and maintenance of instruments
① light source. The life of the light source is limited. In order to prolong the service life of the light source, do not turn on the light source when the instrument is not in use. The switching times should be reduced as far as possible. Lights can be left on during short work intervals. The light source that has just been turned off cannot be restarted immediately. The continuous service time of the instrument should not exceed 3h. If you need to use for a long time, the best intermittent 30min. If the brightness of the light source is obviously weakened or unstable, it should be replaced with a new light in time. After the replacement to adjust the position of the filament, do not contact the window or bulb directly with the hand, to avoid oil contamination. If accidentally touched, wipe with anhydrous ethanol.
② monochromator. The monochromator is the core part of the instrument. It is enclosed in a sealed box and cannot be disassembled. The selected wavelength should be balanced rotation, not too hard. In order to prevent the dispersion element from being affected by moisture and mildew, it is necessary to replace the desiccant (silica gel) of the monochromator box regularly. If the desiccant discoloration is found, it should be replaced immediately.
③ Absorption pool. The absorber cell must be used correctly and special care should be taken to protect the two optical surfaces of the cell.
④ Detector. The photoelectric conversion element can not be exposed for a long time, and should avoid strong light or damp dust.
⑤ When the instrument stops working, the power must be cut off.
In order to avoid dust accumulation and contamination of the instrument, when it stops working, the dust cover should be covered.
If the instrument does not need to power on a regular basis, each time not less than 20 ~ 30min, in order to keep the machine is dry, and maintain the performance of electronic components.