Micro Spectrophotometer maintenance tips
Micro Spectrophotometer is a precision optical instrument, which is carefully assembled and adjusted before leaving the factory. If the instrument can be properly maintained, not only the reliability and stability of the instrument can be guaranteed, but also the service life of the instrument can be extended.
Micro Spectrophotometer is commonly used for quantification of oligonucleotides, single - and double-stranded DNA, and RNA soluble in buffers. The maximum absorption wavelength of nucleic acid is 260 nm. Each nucleic acid has a different molecular composition, so its conversion coefficient is different. To quantify different types of nucleic acids, the corresponding coefficients should be selected in advance. For example, the absorption value of 1OD is equivalent to 50μg/ mL of dsDNA, 37μg/ mL of ssDNA, 40μg/ mL of RNA, 30μg/ mL of OLIG. The absorption value after the test is converted by the above coefficients to obtain the corresponding sample concentration.
Micro Spectrophotometer is a precision optical instrument. Correct installation, use and maintenance play an important role in maintaining the good performance of the instrument and ensuring the accuracy of measurement. Now let's share some common sense of maintenance of the Micro Spectrophotometer.
(3) indoor lighting should not be too strong, and should avoid direct sunlight.
④ The fan should not blow directly to the instrument to prevent the light source lamp from affecting the normal use of the instrument because of the light instability.
(5) Try to stay away from high intensity magnetic field, electric field and high-frequency electrical equipment.
The power supply voltage of the instrument is AC 220V±22V, frequency is 50Hz±1Hz, and must be equipped with a good grounding wire. It is recommended to use electronic AC regulator or AC constant voltage regulator with power of more than 1000W to enhance the anti-interference performance of the instrument.
Avoid use in places with corrosive gases such as hydrogen sulfide.
(2) Routine maintenance and maintenance of instruments
(1) light source. The life of the light source is limited. In order to prolong the service life of the light source, do not turn on the light source when the instrument is not in use. The switching times should be reduced as far as possible. Lights can be left on during short work intervals. The light source that has just been turned off cannot be restarted immediately.
The continuous service time of the instrument should not exceed 3h. If you need to use for a long time, the best intermittent 30min. If the brightness of the light source is obviously weakened or unstable, it should be replaced with a new light in time. After the replacement to adjust the position of the filament, do not contact the window or bulb directly with the hand, to avoid oil contamination. If accidentally touched, wipe with anhydrous ethanol. ② monochromator. The monochromator is the core part of the instrument. It is enclosed in a sealed box and cannot be disassembled. The selected wavelength should be balanced rotation, not too hard. In order to prevent the dispersion element from being affected by moisture and mildew, it is necessary to replace the desiccant (silica gel) of the monochromator box regularly. If the desiccant discoloration is found, it s