Note and application of Micro Spectrophotometer
In colorimetric analysis, the color depth of the solution of the colored substance depends on the intensity of the incident light, the concentration of the solution of the colored substance and the thickness of the liquid layer. When a beam of monochromatic light irradiates the solution, the stronger the intensity of the incident light is, the larger the concentration of the solution is, the thicker the liquid layer is, and the more light is absorbed by the solution. The relationship between them conforms to the quantitative law of light absorption of substances, namely Lambert-Bear law. This is the theoretical basis of ultramicroscopic visible spectrophotometer for quantitative analysis of substances.
Precautions for ultrafine visible spectrophotometer
1. The instrument should be placed in a dry room, placed on a firm and smooth workbench when used, and indoor lighting should not be too strong. In hot days, the fan cannot be used to blow directly to the instrument to prevent the light bulb filament from shining and unstable.
2. Before using the instrument, the user should first understand the structure and working principle of the instrument, the formaldehyde detector and the function of each control knob. Before not press the power supply, the safety performance of the instrument should be checked, the power supply wiring should be firm, the power supply should be good, the starting position of each adjusting knob should be correct, and then press the power supply switch. Abstract: UV-Vis spectrophotometer is one of the most useful analytical tools for analysts. Almost every analytical laboratory cannot do without UV-Vis spectrophotometer. The following introduces the principle, structure and characteristics of ultraviolet spectrophotometer, and introduces its application in the biological field and other applications
Ultramicro visible spectrophotometer is a kind of important analytical instruments, whether in physics, chemistry, biology, medicine, material