Notes of glue recovery and cutting during Electrophoresis

1. The buffer and gel of electrophoresis are newly made, and the gel cutting table should be cleaned and the blade should be cleaned. In a word, the cut band should be guaranteed to be free from foreign DNA pollution.
2. Glue cutting is to recycle all the glue at the location of the whole purpose fragment. In order to reduce the volume of glue, relatively thin glue can be used, as long as enough sample can be used, but also can use a thin and wide comb to run glue.
3 about protection, after the general plexiglass is enough, wear a protective mask to protect your eyes, if you wear resin glasses. If you are very afraid of ULTRAVIOLET irradiation, or have special requirements for the glue, such as the requirement that the glue does not contain EB, point marker and a ruler with scale can be used to take photos together to determine the position of the target strip on the glue for cutting.
4.Point marker gel in accordance with the normal program, then cut off the marker glue, stain with EB, and take photos together with the graduated ruler under uv lamp. Since the relative position between the band to be recycled and marker is known, the position of the target band on the undyed glue can be measured according to the ruler. If it is difficult to judge, a small number of samples can be directly placed on the marker edge points, so that the position can be easily determined.